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. 2024 Sep 6;5(3):103296. doi: 10.1016/j.xpro.2024.103296

4× sample buffer

Reagent Final concentration (mM) Amount
TRIS pH 6.8 240 24 mL
SDS 8% 8 g
Glycerol 40% 40 mL
Bromophenol blue 0.009% 0.009 g
dH2O N/A Make up to 100 mL

Can be kept at RT for at least 1 year. Make 2x sample buffer when needed.