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. 2024 Sep 6;5(3):103296. doi: 10.1016/j.xpro.2024.103296

SDS-PAGE running buffer

Reagent Final concentration Amount
TRIS 25 mM 30.25 g
Glycine 250 mM 187.65 g
SDS 0.1% 10 g
dH2O N/A Make up to 1,000 mL

Can be kept at RT for up to 1 month.