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. 2024 Sep 6;5(3):103296. doi: 10.1016/j.xpro.2024.103296

Feeding media

Reagent Final concentration Amount
Neurobasal medium N/A 480 mL
B27 supplement 2% 10 mL
Glutamax 1% 5 mL
Penicillin-streptomycin 1% 5 mL

Prepare under the hood, keep in fridge and use within 3 months.