Reagent | Final concentration | Amount |
---|---|---|
Neurobasal medium | N/A | 480 mL |
B27 supplement | 2% | 10 mL |
Glutamax | 1% | 5 mL |
Penicillin-streptomycin | 1% | 5 mL |
Prepare under the hood, keep in fridge and use within 3 months.
Reagent | Final concentration | Amount |
---|---|---|
Neurobasal medium | N/A | 480 mL |
B27 supplement | 2% | 10 mL |
Glutamax | 1% | 5 mL |
Penicillin-streptomycin | 1% | 5 mL |
Prepare under the hood, keep in fridge and use within 3 months.