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Interaction of PyV Tag with recombinant pol-prim. BSA (1 μg) (column 1) or purified, recombinant pol-prim complexes (1 μg) (columns 2 to 8, as indicated) were immobilized. After incubation with 1 μg of PyV Tag for 1 h at RT, Tag–pol-prim complexes were detected with Tag-specific antibody F5. The means of three experiments are presented. OD, optical density.
