Table 3.
Detailed description of sequences.
| Patient number | CY | P | Intraoperative peritoneal washing lavage | FFPE | Concordance | pStage | Recurrence | |||
|---|---|---|---|---|---|---|---|---|---|---|
| PNA-directed PCR clamping | Sanger sequence and/or NGS | Estimated VAF (%) | Material | Sanger sequence | ||||||
| Ct | ||||||||||
| 1 | 0 | 0 | 29.086 | GGT > GTT (G12V) | OER | Pancreas | GGT > GTT (G12V) | Yes | 2A | PR |
| 2 | 1 | 1 | 30.249 | GGT > GAT (G12D) | 62.549 | Peritoneum | GGT > GAT (G12D) | Yes | 4 | * |
| 3 | 1 | 1 | 31.801 | GGT > GTT (G12V) | 16.198 | No material | N.A | N.A | 4 | * |
| 4 | 1 | 0 | 33.243 | GGT > CGT (G12R) | 4.616 | No material | N.A | N.A | 3 | * |
| 5 | 0 | 0 | 33.429 | GGT > GAT (G12D) | 3.926 | Pancreas | wt | No | 1A | No |
| 6 | 0 | 0 | 33.591 | GGT > GTT (G12V) | 3.410 | Pancreas | wt | No | 2B | PR, LR, LN |
| 7 | 1 | 1 | 34.103 | GGT > GTT (G12V) | 2.183 | Peritoneum | GGT > GTT (G12V) | Yes | 4 | * |
| 8 | 0 | 0 | 34.544 | wt | 1.487 | Pancreas | GGT > CGT (G12R) | No | 2A | PR, LR |
| 9 | 1 | 0 | 34.590 | GGT > GAT (G12D) | 1.429 | Pancreas | GGT > GAT (G12D) | Yes | 2B | PR, Lung |
| 10 | 0 | 0 | 34.605 | GGT > CGT (G12R) | 1.410 | Pancreas | GGT > CGT (G12R) | Yes | 2B | PR |
| 11 | 0 | 0 | 35.217 | GGT > GAT (G12D) | 0.828 | Pancreas | GGT > GAT (G12D) | Yes | 2A | No |
| 12 | 0 | 0 | 35.486 | wt | 0.655 | Pancreas | GGT > GTT (G12V) | No | 2A | PR, Remnant pancreas |
| 13 | 0 | 0 | 35.734 | wt | 0.528 | Pancreas | GGT > GTT (G12V) | No | 2B | Other |
| 14 | 0 | 0 | 35.837 | GGT > GAT (G12D) | 0.483 | Pancreas | GGT > GAT (G12D) | Yes | 2B | Liver, LN |
| 15 | 0 | 0 | 36.111 | wt | 0.380 | Pancreas | GGT > GAT (G12D) | No | 2B | No |
| 16 | 0 | 0 | 36.133 | wt | 0.373 | Pancreas | GGT > GTT (G12V) | No | 1A | No |
| 17 | 1 | 0 | 36.151 | GGT > GAT (G12D) | 0.367 | Pancreas | GGT > GAT (G12D) | Yes | 2A | No |
| 18 | 0 | 0 | 36.230 | wt | 0.343 | Pancreas | GGT > GAT (G12D) | No | 1A | No |
| 19 | 0 | 0 | 36.264 | wt | 0.333 | Pancreas | GGT > GAT (G12D) | No | 2B | Lung, LR |
| 20 | 0 | 0 | 36.264 | wt | 0.333 | Pancreas | wt | Yes | 2B | Liver |
| 21 | 0 | 0 | 36.310 | GGT > GTT (G12V) | 0.320 | Pancreas | GGT > GTT (G12V) | Yes | 2B | LR |
| 22 | 0 | 0 | 36.420 | GGT > TGT (G12C) | 0.290 | Pancreas | GGT > TGT (G12C) | Yes | 2B | PR, Liver, Lung |
| 23 | 1 | 0 | 36.428 | wt | 0.288 | No material | N.A | N.A | 3 | * |
| 24 | 0 | 0 | 36.550 | wt | 0.259 | Pancreas | GGT > GTT (G12V) | No | 2B | No |
| 25 | 0 | 0 | 36.692 | wt | 0.229 | Pancreas | GGT > GAT (G12D) | No | 2B | No |
| 26 | 0 | 0 | 36.706 | wt | 0.226 | Pancreas | GGT > GAT (G12D) | No | 2A | Lung |
| 27 | 0 | 1 | 36.729 | wt | 0.222 | Pancreas | Insufficient material | N.A | 4 | * |
| 28 | 0 | 0 | 36.747 | wt | 0.219 | Pancreas | GGT > GAT (G12D) | No | 2B | Lung, LR, LN |
| 29 | 0 | 0 | 36.799 | wt | 0.209 | Pancreas | GGT > GTT (G12V) | No | 2B | No |
| 30 | 0 | 0 | 36.842 | wt | OER | Pancreas | GGT > GAT (G12D) | No | 2A | Lung |
| 31 | 0 | 0 | 37.013 | wt | OER | Pancreas | GGT > GTT (G12V) | No | 2B | PR, Liver, LR |
| 32 | 0 | 0 | 37.066 | wt | OER | Pancreas | GGT > GAT (G12D) | No | 2A | No |
| 33 | 0 | 0 | 37.085 | wt | OER | Pancreas | wt | Yes | 2B | No |
| 34 | 0 | 0 | 37.138 | wt | OER | Pancreas | GGT > GAT (G12D) | No | 2A | No |
| 35 | 0 | 0 | 37.383 | wt | OER | Pancreas | GGT > CGT (G12R) | No | 2B | LN |
| 36 | 0 | 0 | 37.660 | wt | OER | Pancreas | GGT > GAT (G12D) | No | 2B | Liver |
| 37 | 0 | 1 | 37.682 | wt | OER | No material | N.A | N.A | 4 | * |
| 38 | 0 | 0 | 37.691 | wt | OER | Pancreas | GGT > GAT (G12D) | No | 2B | PR, Liver, Lung |
| 39 | 0 | 0 | 37.789 | wt | OER | Lymph node | GGT > GAT (G12D) | No | 4 | * |
| 40 | 0 | 0 | 38.053 | wt | OER | Pancreas | GGT > GTT (G12V) | No | 2B | LR |
| 41 | 0 | 0 | 38.799 | wt | OER | Pancreas | GGT > GAT (G12D) | No | 2A | No |
| 42 | 0 | 0 | 38.831 | wt | OER | Pancreas | wt | Yes | 2B | Liver |
| 43 | 0 | 0 | 38.854 | wt | OER | Pancreas | GGT > GAT (G12D) | No | 1A | No |
| 44 | 0 | 0 | 38.952 | wt | OER | No material | N.A | N.A | 3 | * |
| 45 | 0 | 0 | 39.085 | wt | OER | Pancreas | GGT > GAT (G12D) | No | 2B | LR |
| 46 | 0 | 0 | 39.125 | wt | OER | No material | N.A | N.A | 3 | * |
| 47 | 0 | 0 | 39.319 | wt | OER | No material | N.A | N.A | 3 | * |
| 48 | 0 | 0 | 39.337 | wt | OER | No material | N.A | N.A | 2B | No |
| 49 | 0 | 0 | 39.350 | wt | OER | Pancreas | GGT > GAT (G12D) | No | 2A | No |
| 50 | 0 | 0 | 39.604 | wt | OER | Pancreas | GGT > GTT (G12V) | No | 2A | No |
| 51 | 0 | 0 | 40.000 | wt | OER | Pancreas | wt | Yes | 2A | No |
| 52 | 0 | 0 | 40.000 | wt | OER | Pancreas | wt | Yes | 1B | No |
| 53 | 0 | 0 | 40.000 | wt | OER | Pancreas | GGT > GTT (G12V) | No | 2A | No |
| 54 | 0 | 0 | 40.000 | wt | OER | Pancreas | GGT > GAT (G12D) | No | 2A | No |
CY intraoperative peritoneal lavage washing cytology, P peritoneal dissemination at surgery, pfDNA peritoneal floating cell DNA, PNA peptide nucleic acid; PCR: polymerase chain reaction, FFPE formalin-fixed paraffin-embedded, NGS next-generation sequence. VAF variant allele frequency, wt wild type, OER out of estimated range, N.A. not applicable, PR peritoneal recurrence, LR locoregional recurrence, LN extra regional lymph node metastasis.
*: Patients who did not undergo curative resection.