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. 2024 Sep 18;15:8176. doi: 10.1038/s41467-024-52429-9

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Illustration of D1R-Nurr1cKO mouse strain construct (left) and the coronal brain levels that were dissected for bulk RNA sequencing (right). b Volcano plot showing the CLA-enriched genes that were significantly downregulated in D1R-Nurr1cKO mice (Ctrl: n = 4, D1R-Nurr1cKO: n = 4; *p-value adjusted < 0.1, Benjamini & Hochberg correction). c Heatmap showing the expression z-score of the CLA-enriched genes across the different samples. d–k ISH autoradiographs (left) and graphs (right), showing Gnb4 (d), Sstr2 (e), Ntng2 (f), Slc17a6 (g), Bdnf (h), Oprk1 (κOR) (i), 5HT_2AR (j) and 5HT_2CR (k) mRNA at CLA of Ctrl and D1R-Nurr1cKO mice (Ctrl: n = 5, D1R-Nurr1cKO: n = 6; d: *p < 0.0001, e: *p < 0.0001, f: *p < 0.0001, g: *p = 0.0006, h: *p = 0.0046, i: *p < 0.0001, j: *p = 0.0006, k: *p = 0.0004, Two-sided Unpaired t-test). Data in line-graphs are expressed as mean ± SEM. Boxplots show all data points, the 25th and 75th percentile (box), the median (center), and the maxima (whiskers). OD: optic density, arb: arbitrary units, AI: agranular insula, CLA: claustrum/dorsal endopiriform cortex complex, CP: caudoputamen, AUC: area under the curve, FC: fold change, ISH: in situ hybridization. Source data are provided as a Source Data file.