Table 4.

Properties of wild-type and mutant-type amino acids regarding size, charge, and hydrophobicity analyzed by Project HOPE.

AA change	Wild type AA			Mutant type AA
	Size	Charge	Hydrophobicity	Size	Charge	Hydrophobicity
R138Q	Larger	Positive	None	Smaller	Neutral	None
I141N	Smaller	_	More hydrophobic	Larger	_	Less hydrophobic
W136G	Larger	_	More hydrophobic	Smaller	_	Less hydrophobic
I349N	Smaller	_	More hydrophobic	Larger	_	Less hydrophobic
L303R	Smaller	Neutral	More hydrophobic	Larger	Positive	Less hydrophobic
E140A	Larger	Negative	Less hydrophobic	Smaller	Neutral	More hydrophobic
W306C	Larger	_	More hydrophobic	Smaller	_	Less hydrophobic
L308Q	Smaller	_	More hydrophobic	Larger	_	Less hydrophobic

‘_’ indicates neutral effect.