Fig. 4.

Decellularized ECM possesses biologically active cell-adhesion related proteins. A) Schematic of ECM protein absorption on cell-culture plastic surfaces and further ATDC5 cell line seeding, showing initial and 24 h cell-morphology and spreading, B) Confocal images of cells at different surfaces stained for actin cytoskeleton (orange) and Nuclei (DAPI, Blue), C-E) RTCA data, C) quantitative Kinetic cell adhesion on multiple protein-adsorption treated surfaces (The color code indicates the protein adsorbed in each case) (n = 3), D-E) cell adhesion at selected time points, D) at 6 h, E) at 24 h after initial cell-seeding (n = 3). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)