Fig. 3. Loading of the microfluidic chip with the hydrogel, mouse embryonic kidneys and culture medium. A. Loading of the embryonic kidney in fibrin gel into the central channel. B. Loading of the cell culture medium into the lateral channels. C. Attachment of the PDMS thin layer to the chip. D. Attachment of the PDMS round patches to the loading ports. E. General overview of the prototype B microfluidic chip with a mouse embryonic kidney in fibrin gel. F. The mouse embryonic kidney on the porous membrane with holes of 30 μm is shown at higher magnification. Scale bars for A–D: 5 mm; for E: 1 mm; for F: 500 μm.
