Figure 6.

Mutagenesis analysis of key amino acid residue within HhMsrA

(A) Specific activities of HhMsrA-WT and its variants under various concentrations of KCl, each value represents the mean ± SD of three independent experiments.

(B–D) RMSD (B), SASA (C), and Rg (D) comparisons between HhMsrA-WT and the variants HhMsrA-R168K and HhMsrA-R168P.

(E) Total number of salt bridges within variants HhMsrA-R168K and HhMsrA-R168P during simulation.

(F) Structure analysis of salt bridge involving key residue 168 within variants HhMsrA-R168K (red) and HhMsrA-R168P (blue).