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. 2024 Aug 23;27(9):110806. doi: 10.1016/j.isci.2024.110806
REAGENT or RESOURCE SOURCE IDENTIFIER
Bacterial and virus strains
TOP10 Chemically Competent Cell Beijing Tsingke Biotech Co., Ltd. Cat: TSC-C12
BL21 Star (DE3) Chemically Competent Cell Beijing Tsingke Biotech Co., Ltd. Cat: TSC-E01
Chemicals, peptides, and recombinant proteins
50XTAE Buffer Sangon Biotech Cat: B548101-0500
DNA Marker (250∼10000 bp) Sangon Biotech Cat: B600022-0050
Coomassie Brilliant Blue Quick stain Epizyme Cat:PS111
Omni-EasyTM instant protein loading buffer (denatured, reduced, 5 ×) Epizyme Cat:LT101S
Two-color pre-staining protein Marker 10 KDa∼250 KDa Epizyme Cat: WJ102
Oligonucleotides
Primer: R168K Forward:
GAAGGTCGCGAAGGTCAAAG
AGGAGTTCGCCGAG		 This paper N/A
Primer: R168K Reverse:
CTCGGCGAACTCCTCTTTG
ACCTTCGCGACCTTC		 This paper N/A
Primer: R168F Forward:
GAAGGTCGCGAAGGTCTTTG
AGGAGTTCGCCGAG		 This paper N/A
Primer: R168F Reverse:
GAAGGTCGCGAAGGTCTTTGA
GGAGTTCGCCGAG		 This paper N/A
Primer: R168A Forward:
CGAAGGTCGCGAAGGTCGCGG
AGGAGTTCGCCGAGAAG		 This paper N/A
Primer: R168A Reverse:
CTTCTCGGCGAACTCCTCCGCG
ACCTTCGCGACCTTCG		 This paper N/A
Primer: R168E Forward:
GAAGGTCGCGAAGGTCGAAGA
GGAGTTCGCCGAG		 This paper N/A
Primer: R168E Reverse:
CTCGGCGAACTCCTCTTCGAC
CTTCGCGACCTTC		 This paper N/A
Primer: R168P Forward:
GAAGGTCGCGAAGGTCCCGG
AGGAGTTCGCCGAGAAG		 This paper N/A
Primer: R168P Reverse:
CTTCTCGGCGAACTCCTCCGGG
ACCTTCGCGACCTTC		 This paper N/A
Critical commercial assays
Gel Mini Purification Kit Zomanbio Cat: ZP202
Site-directed Mutagenesis Kit Sangon Biotech Cat: B639281-0020
BeyoGold™ His-tag Purification Resin Beyotime Cat: P2218
BCA protein concentration assay kit Beyotime Cat: P0009
Easy PAGE color rapid gel preparation kit (10%) Seven Biotech Cat: SW143-02
Software and algorithms
Origin 2022 Origin https://www.originlab.com/2022
GraphPad Prism 9.5.0 Prism https://www.graphpad-prism.cn/
Lasergene Lasergene https://www.dnastar.com/software/lasergene/
Gromacs 2020.6 Spoel et al.57 https://manual.gromacs.org/2020.6/download.html
Python 3.9 Python Software Foundation https://www.python.org/downloads/release/python-390/
VMD version 1.9.3 Humphrey et al.58 https://www.ks.uiuc.edu/Research/vmd/
PyMOL DeLano et al.59 https://pymol.org/
Autodock Vina Trott et al.60 https://github.com/ccsb-scripps/AutoDock-Vina