Figure 2. Eomes^hiNK^neg cells are distinct from NK cells and ILC1s.

a, Dot plot showing expression of select genes differentially expressed in Eomes⁺Tcf7^int, NK, or ILC1 clusters, relative to the other two clusters in scRNA-seq data. FDR-adjusted P < 0.05; abs. log2FC > 0.5. Dot color, size, as in Fig. 1b. b, Representative flow cytometry plots of Eomes-GFP⁺NK1.1⁻NKp46⁻ cells (Eomes^hiNK^neg) and Eomes-GFP⁺NK1.1⁺NKp46⁺ NK cells in BM CD4⁻CD8⁻CD3ε⁻TCRβ⁻TCRγδ⁻CD19⁻B220⁻Gr1⁻CD11c⁻CD25⁻Ter119⁻(Lin⁻) CD45⁺ cells from Eomes^GFP mice. c, Representative histogram showing the expression of NK associated markers KLRG1, CD11b, DX5, NKG2A/C/E, NKG2D, CD244, CD27, perforin, granzyme B, Ly49H, Ly49C/F/I/H, TCF7, T-bet, L-selectin and IL-7Rα on Eomes^hiNK^neg cells or NK cells from the BM of Eomes^GFP/+Tcf7^mCherry/+ mice. Data are pooled from 2 independent experiments.