Comparison of differentially abundant SAT lipids measured in morbid obese individuals before and after bariatric surgery (cohort 1)
(A) Volcano plot of the differentially abundant lipids between pre- and post-bariatric SAT samples (n = 5). Colored dots highlight significant up- or down-regulated individual lipid species (fold change ≥1.5 and p < 0.05, paired design).
(B) Hierarchical analysis (Distance Measure: Euclidian; Clustering algorithm: Ward) of top 40 SAT lipids with most contrasting patterns between pre- and post-bariatric SAT samples (n = 5).
(C) Lipid class repartition (PC, PE, PI, CL, HexCer, Cer, and SM) in human SAT collected before and after GBS (in mol %).
(D) Relative level changes (mol %) of DHCer, Cer, HexDHCer, and HexCer in human SAT collected before and after GBS.
(E) Venn diagrams assessing the overlap between SAT and serum DALs (fold change ≥1.5 and p < 0.05) in each subject following GBS. Pie diagrams depict the range of lipid classes regulated in both SAT and serum following GBS. Seven lipids were differently regulated in both SAT and serum across all five analyzed individuals. Statistics for (C and D) are paired t test. Data are represented as mean ± SEM. See also Figure S2.