Figure 6. Dual targeting of PD-L1 and IL-33 changed T-cell cellular landscapes. Design of scRNA-seq experiment (A). UMAP of all cell types from four groups (B). UMAP plots showing all CD4⁺ (C) and CD8⁺ (E) T cells from tumor tissues in four groups. Bar plots exhibiting the proportion of CD4⁺ (D) and CD8⁺ (F) T cells within each cluster. Selected significantly enriched pathways in KEGG analyses based on the differentially expressed genes in T cell clusters (G). Gene Ontology in biological function enrichment analysis of the upregulated (H, J) or downregulated (I, K) expressed genes in T cells of anti-PD-L1-sST2 versus atezolizumab group, and anti-PD-L1-sST2 versus combination group, respectively. LLC, lewis lung carcinoma.