FIG. 7.

v-FLIP is translated from a bicistronic ORF 72/71 RNA in vitro. Constructs encoding either the complete second exon of the bicistronic ORF 72/71 RNA (pCMV 72/71; lane 1) or the second exon with a deletion of the 5′ uORF (pCMV 72/71ΔuORF; lane 2) were in vitro transcribed and translated. [³⁵S]methionine-labeled translation products were subsequently analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography. The positions of the v-cyclin and the v-FLIP proteins are indicated by arrows. The identity of the lower band (v-FLIP) was verified by in vitro transcription and translation of constructs in which ORF 71 was deleted or replaced by luciferase (data not shown).