Table 8.
PCR protocols used to screen blood samples from domestic dogs in Chad, Africa
| Pathogen | Gene Target (size in bp) | Primers | Primer Sequence (5’➔ 3’) | Reference |
|---|---|---|---|---|
|
Babesia spp. and Hepatozoon spp. |
18 S rRNA (500 for Babesia, 600 for Hepatozoon) | Primary: 5.1 / B |
CCTGGTTGATCCTGCCAGTAGT TGATCCTTCTGCAGGTTCACCTAC |
[47, 48] |
| Secondary: Babesia F / Babesia R |
GTGAAACTGCGAATGGCTCA CCATGCTGAAGTATTCAAGAC |
|||
| Internal Transcribed Spacer rRNA (ITS) (300 for Babesia, < 200 for Hepatozoon) | Primary: 15 C / 13B |
CGATCGAGTGATCCGGTGAATTA GCTGCGTCCTTCATCGTTGTG |
[50] | |
| Secondary: 15D / 13 C |
AAGGAAGGAGAAGTCGTAACAAGG TTGTGTGAGCCAAGACATCCA |
[51] | ||
| Ehrlichia canis | 16 S rRNA (389) | Primary: ECC / ECB | AGAACGAACGCTGGCGGCAAGCC CGTATTACCGCGGCTGCTGGCA | [52] |
| Secondary: ECA / HE3 |
CAATTATTTATAGCCTCTGGCTATAGG TATAGGTACCGTCATTATCTTCCCTAT |
[52, 53] | ||
| Anaplasma platys | 16 S rRNA (400) | Primary: ECC / ECB |
AGAACGAACGCTGGCGGCAAGCC CGTATTACCGCGGCTGCTGGCA |
[52] |
| Secondary: PLA2 / GA1UR |
TTTGTCGTAGCTTGCTATG- GAGTTTGCCGGGACTTCTTCT |
[54, 55] |