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. 2024 Sep 18;24:319. doi: 10.1186/s12935-024-03505-z

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 6 — NENF was identified metastasis related gene and upregulated in TNBC. A The intersection of genes in module 6 and upregulated genes in metastatic TNBC cells showed by Venn diagram. B The copy number changes of candidate genes in TCGA-TNBC were displayed by heat map. C tSNE scatter plots displayed the expression of NENF in TNBC malignant cells. The survival analysis of TNBC patients with high‑ or low‑NENF expression levels, including OS (D) and RFS (E), depicted by KM curves. F The NENF expression level in normal tissues and TNBC tissues. G The NENF expression level among TNBC stages. H The mRNA expression level of NENF in 30 paired TNBC tissues detected by RT-qPCR. I The protein expression level of NENF in 20 paired TNBC were detected by IHC and the scores of IHC staining. J The standard score of IHC staining. **p < 0.01, ***p < 0.001