FIG. 3.

Analysis of AMV RdRp by glycerol gradient centrifugation. Samples from each gradient fraction were assayed for RdRp activity (A) and analyzed by Western blotting (B to E). RdRp activity was measured in vitro, using plus-strand AMV RNA 3 as the template; a gel run with the radiolabeled minus-strand RNA 3 products is shown. Western blots were analyzed using polyclonal antibodies against AMV proteins P1 (B), P2 (C), and CP (D and E). The antiserum against CP detected CP dimers and monomers, which are separately shown in panels D and E, respectively. Sedimentation is from right to left.