Figure 2.

Overview of the DNA fragments used for assembly of T7 by GG and yeast cells. The wt T7 genome is shown from one direct terminal repeat to the other, indicated as black triangles. Genes of more than 500 bases are illustrated by gray boxes with points indicating orientation. Colored boxes below the wt T7 genome indicate the DNA fragments used in each assembly. Synonymous point mutations introduced in the genome during assembly are indicated by asterisks (*). The darker DNA fragment in the yeast assembly indicates the yeast artificial chromosome (YAC) element inserted between the terminal repeats. The point mutation in the yeast assembly was introduced for identification purposes. Both assemblies were created as circular constructs with fragment 1 joining the last fragment in the assembly (fragment 8 in the GG assembly and the YAC element in the yeast assembly).