Figure 4.

Process for constructing and sequencing the barcoded T7 library for diversity determination. T7 phage DNA was amplified in nine fragments by PCR for GG assembly. A barcode was incorporated in one of the fragments, indicated by a dark gray segment. See Supplementary data for details of barcode design. Assembled circular genomes were subsequently rebooted by cell-free TXTL reactions. The rebooted phages were amplified and DNA was extracted and sequenced by Illumina sequencing. Barcodes were extracted from the sequencing data.