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. 2024 Sep 18;10(38):eadp4995. doi: 10.1126/sciadv.adp4995

Fig. 2. Intein-extein partnerships and intein composition affect the efficiency of intein splicing.

Fig. 2.

(A) Schematic of the maltose-binding protein (MBP)–intein (Int)–GFP (MIG) reporter constructs wherein intein-containing fluorescent precursor proteins must excise the intein and ligate the exteins to generate a matured, smaller fluorescent product (MBP-GFP). (B and C) Native PAGE and quantification of fluorescent signals reveal the impact of intein composition and the presence of the HEN domain on the efficiency of intein excision.