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. 2024 Sep 18;10(38):eadq1476. doi: 10.1126/sciadv.adq1476

Fig. 1. Enriched integrin α4β7 targets HSPC EV uptake into ECs.

Fig. 1.

(A) Workflow for isolation and purification of HSPC-derived vesicles and donor-matched plasma EV controls (n = 3 donors). (B) Principle components analysis of protein cargo identified in HSPC and plasma EVs. (C) Overlap of unique proteins in HSPC EVs from donors 1 to 3 (P1 to P3) and plasma EVs. (D) Enrichment analysis of integrin subunits in HSPC EVs compared to plasma EVs. (E) Proposed model of HSPC EV uptake into ECs; created with BioRender. (F) Workflow for isolation and analysis of murine HSPC-derived EVs. (G) Flow cytometric analysis of integrin α4β7 in murine EVs. (H) Representative confocal microscopy images of HSPC EV uptake into BM ECs. (I) Flow cytometric measurement of CFSE-dyed EV uptake into Vcam-1 KO ECs. LC-MS/MS, liquid chromatography tandem mass spectrometry; DC, differential centrifugation; MFI, mean fluorescence intensity; LT-HSC, long-term HSC. *P < 0.05 and **P < 0.01.