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. 2024 Sep 18;10(38):eadq1476. doi: 10.1126/sciadv.adq1476

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Fig. 4. — (A) Representative confocal images showing CCR2 and CXCR4 localization in isolated HSPCs versus those grown in EC cocultures. (B) Representative high-power images of individual cells analyzed. (C) Calculation of Manders’ overlap coefficients (n = 118 to 120 cells per condition). (D) Representative immunoblot analyses and (E) quantitative analysis of CCR2 in HSPC lysates after CXCR4 immunoprecipitation. *P < 0.05 and ***P < 0.001. ns, not significant; Co-IP, coimmunoprecipitation; DAPI, 4′,6-diamidino-2-phenylindole; IgG, immunoglobulin G.