FIG. 8.

Construction and characterization of recombinants BA71VG-A and BA71VG-B. (A) Diagram of the genomic structure of pathogenic ASFV isolate E70; cell culture-adapted, GUS-containing BA71VG; and recombinant viruses BA71VG-A and BA71VG-B. (B) Southern blot hybridization of BA71VG (lane 1), E70 (lane 2), BA71VG-A (lane 3), and BA71VG-B (lane 4). Viral DNAs were digested with BamHI, electrophoresed, blotted, and hybridized with a ³²P-labeled 16.0-kbp BamHI restriction fragment as a probe. (C) Viral growth in macrophage cell cultures. Primary swine macrophage cell cultures were infected (MOI = 5) with either E70 or BA71V recombinant virus, and at the indicated times, duplicate samples were collected and titrated. These data are the means and standard errors of two independent experiments.