TABLE 2.

Absence of reactivity to HSV following ocular infection of OT2xRAG1^−/− mice

Mouse typea	Mean clinical score (day 10) ± SD	Lymphoproliferation (mean cpm ± SD)b			Mean IFN-γ concn (ng/ml)c ± SD with:
		Responders plus uninfected stimulators	Responders plus HSV stimulators	Responders plus OVA stimulators	UV-HSV	OVA	ConA
OT2xRAG1−/−	2.4 ± 0.5	116 ± 90	110 ± 48	19,680 ± 1,450	<1	6.8 ± 1.3	14.8 ± 2.0
B6	0	270 ± 120	20,590 ± 1,120	280 ± 80	4.9 ± 0.5	<1	16.5 ± 1.5
C57BL/6 SCID	2.9 ± 0.8	210 ± 110	30,897 ± 1,148	180 ± 50	8.8 ± 0.6	<1	18.3 ± 1.4

^aMice (n = 6) were infected with 2 × 10⁶ PFU of HSV-1 RE on scarified corneas and then scored for clinical lesions using the slit-lamp microscope as described previously. The data are represented as the mean clinical scores ± the standard deviations. Ocularly infected C57BL/6 SCID mice reconstituted with B6 HSV immune splenocytes were used as positive control for inducing HSK. 

^bMice (n = 6) were terminated at day 10 postinfection, and spleen and lymph node cells were used in a lymphoproliferation assay as described previously. The data are represented as the means cpm incorporated ± the standard deviations. 

^cSplenocytes (2 × 10⁶ cells/ml) from mice were restimulated in vitro with UV-irradiated HSV (UV-HSV; MOI = 5.0), OVA (10 μg/ml), or ConA (2 μg/ml), and the supernatant was assayed for IFN-γ by ELISA.