TABLE 1.

Comparison of inhibition in NERT and homopolymer RT assays

Inhibitor	Inhibitor concn (μM)	% Inhibitiona by:
		ELISA	NERT
NVP	10	87 ± 12	77 ± 11
	100	99 ± 2	100
PFA	30	98 ± 3	45 ± 5
	300	98 ± 2	100
PAA	70	0 ± 1	0
	700	9 ± 2	0
	2,800	42 ± 4	0
ddATP	10	NTb	71 ± 6
	100	NT	100
ddTTP	10	99 ± 1	70 ± 5
	100	100	100
Ro 5-3335	20	NT	0
	25	6 ± 5	NT
	200	NT	0
	250	19 ± 8	NT
Ro 24-7429	20	NT	32 ± 9
	25	62 ± 16	NT
	200	NT	56 ± 5
	250	84 ± 19	NT
RA	10	16 ± 3	26 ± 9
	100	30 ± 7	44 ± 13
	200	50 ± 4	62 ± 4
Curcumin	12.5	18 ± 10	0
	100	29 ± 12	0
	200	29 ± 13	0
DMSO	∼1% (vol/vol)	0	0

^aData are shown as average ± standard deviation from at least three separate experiments. Percent inhibition was calculated as 100(1 − S_n/S₀), where S_n and S₀ represent the PCR signals in the presence of n and 0 μM drug, respectively. Proximal or intermediate PCR primer pairs were used in all cases. 

^bNT, not tested.