FIGURE 3.

4E‐BP1 is hypophosphorylated in both the skeletal and heart muscle of tumor‐bearing rats. A: 4E‐BP1 quantification, the 4E‐BP1γ isoform was normalized to total 4E‐BP1 to assess changes in protein phosphorylation. B) Quantification of eIF4E, total eIF4E was normalized to Ponceau S. C) Phosphorylated ribosomal protein S6 at S235/236 (p‐RPS6S235/236) was normalized to total RPS6. Representative images of all Western blots (10mg of protein) are accompanied by the corresponding Ponceau S (Pon S) staining. Data are expressed as mean fold‐change from controls ± SD. Statistical significance was determined by a two‐tailed unpaired t‐test, P = significant differences from control.