Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Sep 5;11:1381479. doi: 10.3389/fmed.2024.1381479

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2024 Liu, Ge, Shan, Wang, Yang, Sun and Luo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Inhibition of Sema3A protects podocytes from Adriamycin-induced injury. (A) Western blotting assay showing the levels of Sema3A in Adriamycin-induced podocytes. (B) Levels of Sema3A in Control, AN, Control siRNA (siRNASema3A-NC), and siRNASema3A, demonstrating a decrease in protein levels with siRNASema3A. (C) Cell viability of podocytes treated with AN+siRNASema3A and AN+siRNASema3A + rhSema3A (200 ng/mL in culture medium). (D) Podocyte migration area decreased in the siRNASema3A group compared with that in the AN and AN+siRNASema3A + rhSema3A groups, as analyzed using scratch wound assay. Scale bar: 100 μm. (E) Quantitative analysis of migration rates in (D). **p < 0.01 (n = 3). (F) Podocyte migration decreased in the siRNASema3A group compared with that in the AN and AN+siRNASema3A + rhSema3A groups, as confirmed using the Transwell assay. Scale bar: 50 μm. (G) Quantitative analysis of migrated cells in (E). **p < 0.01 (n = 3). (H) Determination of apoptotic podocytes using flow cytometry. (I) Relative apoptosis rate decreased following Adriamycin+siRNASema3A and Adriamycin +siRNASema3A + rhSema3A (200 ng/mL in culture medium) treatments compared with that after AN treatment (n = 3) **p < 0.01.