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TRPC5 expression increased following Sema3A overexpression in podocytes. (A) Western blotting to assess TRPC5 levels in podocytes. (B) Upregulated TRPC5 expression in response to exogenous Sema3A (200 ng/mL) in the culture medium (n = 3) *p < 0.05. (C) Podocytes overexpressing Sema3A. (D) Relative mRNA expression of Sema3A and TRPC5 (n = 3) **p < 0.01. (E) Relative densities of Sema3A and TRPC5 protein expression normalized to GAPDH (n = 3) **p < 0.01.
