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. 2024 Sep 18;15:8212. doi: 10.1038/s41467-024-52510-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 6 — a Schematic diagram of the chromosomes. The donor fragment is shown in red. b Phenotype of the seedling vigor, chilling tolerance, and whole plant for ZF802 and NIL-vig1a. Scale bars, 1 cm left (b), 4 cm upper and middle right (b), and 15 cm bottom right (b). c–h Investigation of the shoot length (c) (n = 20 independent seedlings), seminal root length (d) (n = 20 independent seedlings), survival rate (e) (n = 3 biologically independent experiments), plant height (f) (n = 20 independent plants), grain number per panicle (g) (n = 20 independent panicles), and grain yield per plant (h) (n = 20 independent plants). Values are the mean ± SD (two-tailed t-test). The chilling tolerance for ZF802 and NIL-vig1a was repeated three times with 80-160 seedlings per biological replicate and obtained similar results. Source data are provided as a Source Data file.