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. 2024 Sep 18;7:1172. doi: 10.1038/s42003-024-06850-x

Table 1.

Sequences of the primers used in the quantitative RT-qPCR

Gene Name Upstream primer sequences (5′-3′) Downstream primer sequences (5′-3′)
TNF-α GCGACGTGGAACTGGCAGAAG GCCACAAGCAGGAATGAGAAGAGG
INF-γ TCATGGCTGTTTCTGGCTGTTACTG GACGCTTATGTTGTTGCTGATGGC
IL-6 CTTCTTGGGACTGATGCTGGTGAC CTCTCTGAAGGACTCTGGCTTTGTC
IL-10 TGGAGCAGGTGAAGAGTGAT ATTCATGGCCTTGTAGACACC
IL-4 TGGATGTGCCAAACGTCCTC AGCACCTTGGAAGCCCTACA
PCNA GAGAGCTTGGCAATGGGAACA ACGTTAGGTGAACAGGCTCATT
Ly-6G CCTTCTCCCAGGATGGACAC AATTGTAGCACTCCAGCCCC
Caspase-2 ATGGTGATGGTCCTCCCTGTCTTC CTGTGCGGTCTGGTCATGTAGC
Caspase-3 AGCTTGGAACGGTACGCTAA CCACTGACTTGCTCCCATGT
Caspase-9 ACAGAGAAGGGGCTTGGG GCAATAGCATAAAGACAACTCTGG
Caspase10 GTCAAGTTTGCCTACCCCCA TTCGCAGAAACAGCATTGGC
MCM7 ACCTACCAGCCAATCCAGTCTCC GAGTAAGCCCTGTGCCATCTGTTG
Ki-67 ACACAGAGCCTTAGCAATAGCAACG GCCAGTAACACGAGAGTCTTCATCC
CyclinD1 TCTCCTGCTACCGCACAAC TGGAGGGGGTCCTTGTTTAG
ZO-1 GAAGGCGGATGGTGCTACAAGTG AGGCTCAGAGGACCGTGTAATGG
Occludin AGTCCACCTCCTTACAGACCTGATG GCCTCCATAGCCACCTCCGTAG
GAPDH GGTTGTCTCCTGCGACTTCA TGGTCCAGGGTTTCTTACTCC