Table 1.
Primary and secondary antibodies used in this study
| Target | Source | Cat# | Dilution |
|---|---|---|---|
| Primary antibodies | |||
| Biotin-labeled 6E10 | BioLegend | #803007 | 1:200 |
| Tau13 | Abcam | ab19030 | 1:200 |
| AT8, Ser202/Thr205 | Thermo fisher Scientific | MN1020 | 1:200 |
| AT180, Thr231/Ser235 | Thermo fisher Scientific | MN1040 | 1:200 |
| Anti-NTPDase II | CHUQ | mN2-36LI6 | 1:1,000 |
| Anti-Trpm5 | In house | N/A | 1:500 |
| Anti-Car4 | R&D Systems | AF2414 | 1:1,000 |
| Secondary antibodies | |||
| Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 488 | Thermo fisher Scientific | A32731 | 1:1,000 |
| Goat anti-Guinea Pig IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 | Thermo fisher Scientific | A11073 | 1:1,000 |
| Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 555 | Thermo fisher Scientific | A32727 | 1:1,000 |
| Goat anti-Rabbit IgG (Heavy chain), SuperclonalTM Recombinant Secondary Antibody, Alexa FluorTM 647 | Thermo fisher Scientific | A27040 | 1:1,000 |
| Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 | Thermo fisher Scientific | A21202 | 1:1,000 |
| Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 555 | Thermo fisher Scientific | A32816 | 1:1,000 |