FIG. 5.
Electrophoretic analysis of viral structural proteins of wt and D-EA reoviruses after treatment with cathepsin D. (A) Purified 35S-labeled virions of reovirus strains T3D, D-EA1, and D-EA3 were treated with cathepsin D (pH 3.8) at the concentrations shown at 37°C for 1 h. Equal numbers of viral particles were loaded into wells of 10% polyacrylamide gels. Viral proteins are labeled on the right. (B) Quantitation of ς3 band intensity. Densities of bands corresponding to the ς3 and ς2 proteins were determined, and the results are presented as the ς3/ς2 ratios.