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. 2001 Apr;75(7):3197–3206. doi: 10.1128/JVI.75.7.3197-3206.2001

TABLE 1.

Growth of T1L × D-EA1 reassortant viruses in the presence and absence of E64

Virus strain Origin of gene segmenta:
Viral titer inb:
L+E64/L ratioc Rankd
L1 L2 L3 M1 M2 M3 S1 S2 S3 S4 E64-treated cells Untreated cells
Parental
 T1L L L L L L L L L L L 4.18 × 105 2.20 × 107 0.0190
 D-EA1 E E E E E E E E E E 2.70 × 107 9.91 × 107 0.273
Reassortant
 1-31 L L L L L L E L E E 4.80 × 106 1.20 × 107 0.400 1
 1-6 E E L L E L L E L E 1.05 × 106 8.28 × 106 0.127 2
 35 E E E E L E L E E E 2.55 × 106 2.20 × 107 0.116 3
 1-57 L L L L E L L E E E 1.35 × 106 1.21 × 107 0.112 4
 1-29 E E E E E E L E E E 1.17 × 106 1.40 × 107 0.0836 5
 33 E E E L E E L E E E 2.40 × 105 2.90 × 106 0.0828 6
 1-25 E E E L E E L E E E 1.32 × 106 1.90 × 107 0.0692 7
 1-28 E E L E E E E E L E 3.35 × 106 5.30 × 107 0.0632 8
 29 E E E E E E L E E E 6.85 × 105 1.19 × 107 0.0576 9
 1-1 E E E E E E L E L E 7.75 × 105 2.30 × 107 0.0337 10
 1-11 E E E L E L L E E L 4.53 × 105 1.75 × 107 0.0259 11
 1-9 E E E L E L L E E L 4.08 × 105 1.63 × 107 0.0251 12
 1-56 E E E E L L L E E L 2.25 × 104 1.05 × 106 0.0214 13
 1-10 L L L L L E L L E L 2.95 × 105 2.20 × 107 0.0134 14
 47 E E E L E E L E E L 3.15 × 104 2.85 × 106 0.0111 15
 1-27 E E E E E E L E E L 9.25 × 104 1.03 × 107 0.00902 16
 1-30 E E L E E E E L E L 4.00 × 105 5.40 × 107 0.00741 17
 22 L E L E E E L E E L 8.05 × 104 1.33 × 107 0.00608 18
 1-44 E E L E E E L E E L 3.80 × 104 1.23 × 107 0.00309 19
 37 E E E L E E E E E L 4.95 × 104 2.15 × 107 0.00230 20
a

Parental origin of each gene segment: L, gene segment derived from T1L; E, gene segment derived from D-EA1. 

b

L cells (4 × 105) were infected with virus strains at an MOI of 2 PFU per cell. After a 1-h adsorption period, the inoculum was removed, fresh medium with or without 100 μM E64 was added, and cells were incubated at 37°C for 24 h. Cells were frozen and thawed twice, and viral titers in cell lysates were determined by plaque assay. The results are presented as mean viral titer at 24 h for two to four independent experiments for the reassortant viruses and six independent experiments for the parental viruses. 

c

The mean viral titer at 24 h in E64-treated cells was divided by that in untreated cells to calculate an L+E64/L ratio for each virus strain. 

d

Reassortant viruses are ranked from highest to lowest based on their L+E64/L ratios.