FIG. 4.

RNA2 nt 1 to 882 contain sequences required for 1a-induced membrane association. (A) Expression cassettes for wt RNA2 and RNA2 deletion derivatives are diagrammed at the left. Membrane association of these RNAs with or without 1a was assessed by cell fractionation as described in the legend to Fig. 3, and representative Northern blots are shown at the right. Sup., supernatant. (B) As a quantitative measure of 1a responsiveness, the ratio of RNA accumulation in the membrane-associated pellet fraction in the presence and absence of 1a was calculated for each RNA. Averages and standard errors from three or more independent experiments are shown.