Table 1. Optical Conditions and Calculated S/N Ratios of Raman Spectra Obtained from Two E. coli K12 Colonies.
spatial
resolution [μm] |
S/N ratio
[-] |
|||||
---|---|---|---|---|---|---|
NA | XY | Z | 750 cm–1 | 1130 cm–1 | 1580 cm–1 | 1660 cm–1 |
0.90 | 1.2 | 6.4 | 5.28 | 8.15 | 6.12 | 4.65 |
0.75 | 2.3 | 7.8 | 3.49 | 4.16 | 1.90 | 2.27 |
0.65 | 2.8 | 8.6 | 2.44 | 3.25 | 1.90 | 1.92 |
0.25 | 11 | ∼240 | 2.13a | 1.88a | 1.52a | 1.56a |
For N = 1 owing to the weak signal.