Figure 2.

Design and evaluation of PKS assembly lines. (A) The Plt P1 PKS. The nonfunctional KR domain is labeled KR0. (B) P2 PKS in which the module-1 AT has been inactivated and FabD is added. Product yields are evaluated relative to the stoichiometry of the 4,5-dichloropyrrolyl-SNAC substrate added to the assay. (C) Cal C1 PKS. (D) The C1 and C2 PKSs. No difference in yield of 2 is observed between C1 and C2 PKSs. (E) Competition experiment between Mal and MeMal extender units leading to products 1–4. (F) Yields of 1–4 produced in the competition assays. (G) Assay design and relative amounts of diketides 5 and 6 produced by the four unimodular PKSs. Time-dependent formation of 5 and 6 by (H) P2 and (I) C2 PKSs.