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Expanded competition experiment. (A) Assay design in which four extender units are provided to the P1–P2 and C1–C2 PKSs. Pyrones 7 and 8 collectively represent products in which the PKS module-1 incorporates the cognate extender unit while pyrones 9 and 10 collectively represent molecules in which module-1 incorporates a noncognate extender unit. Product profiles and yields, relative to the 4,5-dichloropyrrolyl-SNAC substrate, for (B) P1 and P2, and (C) C1 and C2 PKSs.
