Fig. 3.

Trem2-deficiency alters striatal glial numbers and shifts oligodendrocyte populations to predominantly non-mature cells. (A) Representative fluorescent images of microglia (IBA1; red) and astrocytes (GFAP; green) and (B) of total oligodendrocytes (Olig2; green) and mature oligodendrocytes (CC1; red) in the striatum of 12- and 18-month-old B6 and Trem2^−/− mice (40x). (C-D) Quantification of microglia and astrocytes in 12- and 18-month-old B6 and Trem2^−/− mice (n = 4). Number (C) of IBA1⁺ microglia and percent area (D) of GFAP⁺ astrocytes per FOV. (E-F) Quantification of oligodendrocytes in 12- and 18-month-old B6 and Trem2^−/− mice (n = 4). Number of Olig2⁺CC1⁻ cells (E) and Olig2⁺CC1⁺ cells (F) for oligodendrocyte precursor and mature cells, respectively. Two-way ANOVA with Tukey’s post-hoc test (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001) was used for statistical analysis. Error bars represent SEM