Fig. 4.

Trem2-deficiency promotes dysfunctional signaling associated with impaired differentiation and maturation during oligodendrogenesis in the striatum. (A) Illustration of general steps in oligodendrogenesis and examples of cytokines and tropic factors involved. (B-I) Analyses of expression levels of oligodendrogenesis-associated cytokines and trophic factors in 6-, 12-, and 18-month-old B6 and Trem2^−/− mice. (B-D) Quantification of signaling involved in migration. Multiplex ELISA analysis of CXCL1 and IL-1β (n = 8–12 mice) and qPCR analysis of Cxcl12 (n = 5–6 mice). (E-G) Quantification of signaling involved in proliferation. Multiplex ELISA analyses of TNF-α, IL-5, and IL-6 (n = 8–12 mice). (H-J) Quantification of signaling involved in differentiation and maturation. ELISA analyses of BDNF, IGF-1, and Gal-3 (n = 5–6 mice). Two-way ANOVA with Tukey’s post-hoc test (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001) was used for statistical analysis. Error bars represent SEM