Fig. 4.

Sand flies have two conserved orthologs of CYP4G17, an important enzyme involved in cuticular hydrocarbon biosynthesis. a) Phylogenetic comparison of CYP4G17 orthologs in 11 phlebotomine species with A. gambiae (Agam) as reference. b) Violin plots of mean log10-transformed normalized expression values (transcripts per million) of total CYP genes on each of the 11 sequenced phlebotomines CYP4G346 orthologs are consistently the most highly expressed CYP genes in each species, while CYP4G347 orthologs are much less expressed. This indicates that among the two, CYP4G346 is the functional ortholog of CYP4G17 in sand flies.