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KD1-SPB DNA is synthesized efficiently in H441 but not Hep3B cells. H441 or Hep3B cells were infected with 10 PFU of KD1 or KD1-SPB/cell. Total genomic DNA was isolated at 0, 5, 24, 48, 72, and 96 h p.i.; digested with HindIII; resolved by agarose gel electrophoresis; blotted; and hybridized with 32P-labeled Ad DNA. (A) Autoradiogram. (B) PhosphorImager quantitation of the DNA bands in panel A.
