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Coimmunofluorescence assay results showing that, whereas KD1-SPB infects a variety of human cancer cell types, it expresses E4ORF3 efficiently only in H441 cells. Cells were infected with 10 PFU of KD1-SPB or KD3/cell. At 44 h p.i., cells were fixed and double stained using a rabbit polyclonal antibody to E4ORF3 and a pool of mouse monoclonal antibodies to E1A. The same fields are shown for E4ORF3 and E1A.
