Fig 3. Correlation between Wolbachia density and viral load of the DENV-1 strain in Aedes albopictus mosquitoes from Reunion Island.
The mosquitoes tested belong to the populations of F0 generation from Sainte-Marie (F0_SM), Saint-Gilles les Hauts (F0_SG), Saint-Philippe (F0_SPh), or Saint-André (F0_SA), and were collected 21 or 28 days post-exposure (dpe) to the DENV-1 local strain. (A.) The total Wolbachia density (wAlbTot i.e. wAlbA + wAlbB) is given based on the ratio between the Wolbachia genomes (wAlbA and wAlbB) and Ae. albopictus genomes (RSP7 concentrations) in the bodies of mosquitoes according to their population of origin. (B.) Number of DENV-1 RNA copies in the body of the mosquitoes according to their population of origin. In graphs A and B, the line inside each boxplot represents the median and the diamond corresponds to the mean of each population. (C.) Number of DENV-1 RNA copies according to the total Wolbachia density in the body of each mosquito tested (N = 43). *p < 0.05, **p < 0.01, Dunn’s tests.