Fig 3. Broadly neutralizing responses in immunized cows are directed to the V2-apex epitope on HIV Env.
(A) Sera from cow-485 and cow-488 over five time points were tested for competition against five well characterized antibodies spanning four HIV bnAb epitopes. Vertical dotted lines represent prime and boost dates. Immunizations of MT145K SOSIP are shown in red, C108 SOSIP in blue, and the SOSIP cocktail in purple. The results are displayed both graphically and tabulated percent breadth. CAP256-VRC26.09 is referred to as VRC26.09. (B) Neutralization ID50 titers are shown for IgG-purified sera tested for neutralization of wild-type and V2-apex epitope mutant viruses spanning multiple time points. NT = not tested. (C) Comparisons of the amino acid CDRH3 lengths for unenriched day 359 and sort-enriched (see text) samples from cow-485 and cow-488 are shown in a pie chart. The percentage of the whole is indicated in each section of the pie. Short CDRH3 length antibodies are shown in yellow, long in pink, and ultralong in purple.