Fig 5. Broadly cow V2-apex neutralizing antibodies have ultralong CDRH3s and have moderate neutralization breadth and relatively high potency.

(A) CDRH3 sequence alignment is shown for Bess and ElsE antibodies compared to germline and NC-Cow1. The sequences for the germline VH1–7, DH8-2, and JH10 regions are shown at the top. CDRH3 lengths (L) are shown to the right. Cysteines within the CDRH3s are highlighted in blue. Negatively charged D and E amino acids are shown in red. (B) Summary results of Bess and ElsE series mAb neutralization measured against a 12-virus global panel. Neutralizing geomean IC₅₀ and geomean IC₈₀ titers are shown for viruses neutralized at IC₅₀ <50 μg/ml. The table also includes percent breadth and the geomean maximum percentage neutralized (MPN) for viruses neutralized at IC₅₀ <50 μg/ml. PGDM1400 and CAP256-VRC26.25 CATNAP data percent breadth, geomean IC₅₀, and geomean IC₈₀ are shown for comparison. C) MAbs Bess1, Bess2, Bess4, ElsE1, and ElsE2 were analyzed for neutralization breadth and potency on 98 viruses of a muti-clade pseudovirus panel. Neutralization breadth and geomean IC₅₀ values are grouped by virus clade.