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. 2024 Sep 19;19(9):e0310524. doi: 10.1371/journal.pone.0310524

Fig 1. BBR reduced intracellular AAT level in Huh7.5Z cells.

Fig 1

(A) MTT-based cell viability assay. Huh7.5 and Huh7.5Z cells were treated with 0 (served as control), 1, 10 and 40 μM of berberine chloride for 48 hours (n = 3). Cell viability (%) = (Mean OD sample / Mean OD Control) ×100. (B, D) Intracellular AAT load. Huh7.5Z cells were treated with 0, 1.2, 2.5, 5, and 10 μM of berberine chloride for 48 hours (n = 3), and western blot was performed to measure intracellular AAT. (B) Representative western blot and (D) quantification graph. (C, E, F, G) AAT transcription, degradation, and secretion. Huh7.5Z cells were treated with 2.5 μM of berberine chloride for 48 hours (n = 3), and western blot was performed for detection of p62. (C) Representative western blot and (E) quantification graph. (F) SERPINA1 gene expression was measured by qPCR, and (G) AAT concentration in culture medium was measured by ELISA. BBR: berberine. MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide. qPCR: quantitative polymerase chain reaction. ELISA: enzyme-linked immunosorbent assay. SERPINA1: Serine protease inhibitor clade A member 1 gene (encoding alpha-1 antitrypsin).