FIG. 4.

MV replication and pathogenesis in macrophage-depleted mouse tissues. (A to E) Histological analysis of macrophage-depleted spleens 3 days after i.p. infection with MV-Ed. (A) Area in the white pulp positive for MV N mRNA, rp, red pulp; wp, white pulp. (B) Syncytia stained with the CD11c cell marker. (C and D) Colocalization of the NLDC145 cell marker (C) with a CD4 T-cell marker (D) within a syncytium (asterisk) located in the central periarterial sheath, CA, central artery. (E) Newly formed PNA-positive germinal centers (red) observed at 3 days p.i. within the white pulp. (F to K) Lung pathology after i.n. administration of clodronate liposomes and MV-Ed. (F) Local depletion of F4/80⁺ macrophages (arrows indicate apoptotic cells). (G) Extensive infiltration of CD11c⁺ DC in the same tissue block. (H to K) Development of MV-induced syncytia that contain either CD11c⁺ (H) and NLDC145⁺ (I) DC or F4/80⁺ (J) and MOMA1⁺ (K) macrophages. Magnifications, ×405 (A to D, H, and J); ×101 (E to G); and ×1,013 (K).