Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | Trp53 | GenBank | ENSMUSG00000059552 | |
| Gene (M. musculus) | Ackr4 | GenBank | ENSMUSG00000079355 | |
| Gene (M. musculus) | Mt2 | GenBank | ENSMUSG00000031762 | |
| Gene (Homo sapiens) | ACKR4 | GenBank | ENSMUSG00000129048 | |
| Strain, strain background (M. musculus, both sexes) | Trp53ΔAS, C57Bl/6J | Simeonova et al., 2013 | ||
| Strain, strain background (M. musculus, both sexes) | Eμ−Myc, C57Bl/6J | Jackson Labs | B6.Cg-Tg(IghMyc)22Bri/J | |
| Strain, strain background (M. musculus, females) | CD-1 Nude CD-1 |
Charles River Labs | Crl:CD1-Foxn1nu | |
| Strain, strain background (M. musculus, both sexes) | C57Bl/6J | Charles River Labs | ||
| Cell line (M. musculus, both sexes) | WT, Trp53+/ΔAS, Trp53ΔAS/ΔAS, Trp53+/-, Trp53-/- fibroblasts | This paper | Primary fibroblasts prepared from E13.5 days embryos | ‘Cells and cell culture reagents’ |
| Cell line (H. sapiens, male) | MRC5 | Sigma-Aldrich | MRC5 PD19 (#05072101) | |
| Cell line (H. sapiens, male) | MRC5-SV40 | Sigma-Aldrich | MRC5-SV2 (#84100401) | |
| Cell line (H. sapiens, female) | HEK293T | ATCC | CRL-3216 | |
| Cell line (H. sapiens, male) | Raji | ATCC | CCL-86 | |
| Cell line (H. sapiens, male) | Raji ACKR4 KO | This paper | ACKR4 KO 4.14 & 5.2 Raji derivatives | Figure 4—figure supplement 3 |
| Transfected construct (Adenoviral E1A) | pWZL-E1A12S | Addgene | pWZL hygro 12S E1A (#18748) | |
| Transfected construct (human Ras) | pBabe-Hrasv12 | Addgene | pBabe-puro Ras v12 (# 1768) | |
| Antibody | p53 (rabbit polyclonal) | Novocastra | Leica NCL-p53-CM5p | 1/2000 |
| Antibody | Myc (mouse monoclonal) | Santa Cruz | 9E-10 sc40 | 1/1000 |
| Antibody | p21 (mouse monoclonal) | Santa Cruz | F-5 sc6246 | 1/200 |
| Antibody | Actin (mouse monoclonal) | Santa Cruz | Actin-HRP sc47778 | 1/5000 |
| Antibody | CD45R/B220 APC (rat, monoclonal) | BD Biosciences | Anti-mouse CD45R/B220 APC (#561880) | 1/200 |
| Antibody | IgD (rat, monoclonal) | BD Biosciences | Anti-mouse IgD BV 605 (#563003) | 1/100 |
| Antibody | CD43 (rat, monoclonal) | BD Biosciences | Anti-mouse CD43 FITC (#561856) | 1/200 |
| Antibody | IgM (rat, monoclonal) | BD Biosciences | Anti-mouse IgM PE (#562033) | 1/50 |
| Recombinant DNA reagent | pSpCas9(BB)–2A-Puro | Addgene | PX459 (#48139) | |
| Sequence-based reagent | Trp53α-F | This paper | qPCR primer | AAAGGATGCCCATGCTACAGA; Figure 1—figure supplement 1 |
| Sequence-based reagent | Trp53α-R | This paper | qPCR primer | TCTTGGTCTTCAGGTAGCTGGAG; Figure 1—figure supplement 1 |
| Sequence-based reagent | Trp53AS-F | This paper | qPCR primer | AAAGGATGCCCATGCTACAGA; Figure 1—figure supplement 1 |
| Sequence-based reagent | Trp53AS-R | This paper | qPCR primer | TGAAGTGATGGGAGCTAGCAGTT; Figure 1—figure supplement 1 |
| Sequence-based reagent | Ackr4-F | This paper | qPCR primer | GCACCTCTCCCAGCTTAAACA; Figure 3 |
| Sequence-based reagent | Ackr4-R | This paper | qPCR primer | AATAGTATTCCGCTGACTGGTTCAG; Figure 3 |
| Sequence-based reagent | ACKR4-F | This paper | qPCR primer | ACTGCTCCTCTCTGCCGACTAC; Figure 4 |
| Sequence-based reagent | ACKR4-R | This paper | qPCR primer | GCCATTCATTTCATTTTCCTCAT; Figure 4 |
| Sequence-based reagent | ACKR4-g4 | This paper | Guide for CRISPR #4 | TGGTAGTGGCAATTTATGCC; Figure 4—figure supplement 3 |
| Sequence-based reagent | ACKR4-g5 | This paper | Guide for CRISPR #5 | GGGCTGTTAATGCAGTTCAT; Figure 4—figure supplement 3 |
| Peptide, recombinant protein | CCL21 | Preprotech | #300-35A | |
| Peptide, recombinant protein | Superscript IV | Invitrogen | TF #18090010 | |
| Commercial assay or kit | Nucleospin RNA II | Macherey-Nagel | FS #NZ74095520 | |
| Commercial assay or kit | Power SYBR Green | Applied Biosystems | # 4367659 | |
| Commercial assay or kit | Supersignal West Femto | Thermo Fisher | # 34096 | |
| Commercial assay or kit | AnnexinV-FITC apoptosis staining/ detection kit | Abcam | # Ab14085 | |
| Commercial assay or kit | Truseq stranded Total RNA | Illumina | #20020596 | |
| Commercial assay or kit | Nucleofector Amaxa kit V | Lonza | # VCA-1003 | |
| Chemical compound, drug | Doxorubicin | Sigma-Aldrich | # D1515 | |
| Chemical compound, drug | Etoposide | Sigma-Aldrich | # E1383 | |
| Chemical compound, drug | Nutlin 3a | Sigma-Aldrich | # SML-0580 | |
| Chemical compound, drug | 17β-estradiol | Sigma-Aldrich | # E2758 | |
| Software, algorithm | FlowJo | Beckton-Dickinson | RRID:SCR_008520 | v 10.10 |
| Software, algorithm | featureCounts | Liao et al., 2014 | ||
| Software, algorithm | DESeq2 R package | Love et al., 2014 | ||
| Software, algorithm | GSEA software | Subramanian et al., 2005 | ||
| Software, algorithm | PWMScan | Ambrosini et al., 2018 | ||
| Software, algorithm | CRISPOR | Haeussler et al., 2016 | ||
| Software, algorithm | Prism | GraphPad | RRID:SCR_002798 | v 5.0 |