Fig 4. Genomic structure of the bovine ALX4 gene and positions of variants detected in Galloway cattle.

The genomic structure of the bovine ALX4 gene as deduced from AC_00172 (Bos_taurus_UMD_3.1), NC_007313 (Btau_4.6.1), and NM_001030304 is depicted [33]. Sizes of intron 1 and 2 are not yet known due to larger gaps. Numbering of positions refers to AC_000172. Duplicated sequences in exon 2 and exon 4 in the affected animals are shown with gray bars. Numbers below the sequences indicate the corresponding nucleotide positions of the wildtype (wt_ex2, wt_ex4) and mutated (mut_ex2dup, mut_ex4dup) alleles within the corresponding exon. Positions of the SNPs and duplications according to HGVS [35] are listed in Table 2.